Integrated bioinformatic pipeline using whole-exome and RNAseq data to identify germline variants correlated with cancer

STAR Protocols

Aakrosh Ratan¹  Ajay Chatrath²  Divya Sahu³  Anindya Dutta³ 

[1] Center for Public Health Genomics, University of Virginia, Charlottesville, VA 22908, USA;Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22903, USA;Department of Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, USA;

关键词: Bioinformatics;    Cancer;    Genetics;    Genomics;    Sequencing;    RNAseq;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Germline Variants (GVs) are effective in predicting cancer risk and may be relevant in predicting patient outcomes. Here we provide a bioinformatic pipeline to identify GVs from the TCGA lower grade glioma cohort in Genomics Data Commons. We integrate paired whole exome sequences from normal and tumor samples and RNA sequences from tumor samples to determine a patient’s GV status. We then identify the subset of GVs that are predictive of patient outcomes by Cox regression.For complete details on the use and execution of this protocol, please refer to Chatrath et al. (2019) and Chatrath et al. (2020).

【 授权许可】

Unknown