Generation of RNA sequencing libraries for transcriptome analysis of globin-rich tissues of the domestic dog

STAR Protocols

Shintaro Katayama¹  Juha Kere²  Masahito Yoshihara³  Kaarel Krjutskov³  Carsten Daub⁴  Hannes Lohi⁵  Sini Ezer⁵ 

[1] Corresponding author;Department of Biosciences and Nutrition, Karolinska Institutet, 14183 Huddinge, Sweden;Stem Cells and Metabolism Research Program, University of Helsinki, 00014 Helsinki, Finland;Department of Biosciences and Nutrition, Karolinska Institutet, 14183 Huddinge, Sweden;Folkhälsan Research Center, 00290 Helsinki, Finland;

关键词: Bioinformatics;    Sequence analysis;    Genomics;    Sequencing;    RNAseq;    Model Organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: We have developed a protocol for barcoded cDNA libraries of 48 samples to study gene expression across tissues in the domestic dog, Canis familiaris, by modifying the Single-Cell Tagged Reverse Transcription (STRT) protocol (Islam et al., 2012, 2014). The cDNA reads represent mRNA 5′ ends, enabling the study of transcription start sites (TSS). Our modifications include longer UMIs for molecular counting and Globin-Lock® to deplete globin mRNAs that are abundant in blood and blood-rich tissues dominating all reads.

【 授权许可】

Unknown