An integrative approach to energy, carbon, and redox metabolism in the cyanobacterium Synechocystis sp. PCC 6803

Vermaas, Willem F. J.

Arizona State University

关键词: Genomics;    Hydrogen Production;    Biomass;    Membranes;    Chloroplasts;   

DOI  :  10.2172/914574
RP-ID  :  DOE ER15251 final
RP-ID  :  FG03-01ER15251
RP-ID  :  914574

美国|英语

来源: UNT Digital Library

【 摘 要 】

The broader goal of this project was to merge knowledge from genomic, metabolic, ultrastructural and other perspectives to understand how cyanobacteria live, adapt and are regulated. This understanding aids in metabolic engineering and synthetic biology efforts using this group of organisms that contribute greatly to global photosynthetic CO2 fixation and that are closely related to the ancestors of chloroplasts. This project focused on photosynthesis and respiration in the cyanobacterium Synechocystis sp. PCC 6803, which is spontaneously transformable and has a known genome sequence. Modification of these fundamental processes in this organism can lead to improved carbon sequestration and hydrogen production, as well as to generation of high-quality biomass. In our GTL-supported studies at Arizona State University we focus on cell structure and cell physiology in Synechocystis, with particular emphasis on thylakoid membrane formation and on metabolism related to photosynthesis and respiration. Results on (a) thylakoid membrane biogenesis, (b) fluxes through central carbon utilization pathways, and (c) distribution mechanisms between carbon storage compounds are presented. Together, these results help pave the way for metabolic engineering efforts that are likely to result in improved solar-powered carbon sequestration and bioenergy conversion. Fueled by the very encouraging results obtained in this project, we already have attracted interest from major companies in the use of cyanobacteria for biofuel production.