Increased Antibacterial and Antibiofilm Properties of Silver Nanoparticles Using Silver Fluoride as Precursor

Molecules

Yuri Diaz Fernandez¹  Daniele Merli²  Chiara Milanese²  Piersandro Pallavicini²  Agnese D’Agostino²  Lorenzo De Vita²  Angelo Taglietti²  Federico Bertoglio³  Livia Visai³  Silvia Rossi⁴  Andrea Falqui⁵  Alberto Casu⁵ 

[1] Department of Chemistry, University of Liverpool, Liverpool L69 3BX, UK;Dipartimento di Chimica, Università di Pavia, viale Taramelli 12, 27100 Pavia, Italy;Dipartimento di Medicina Molecolare, Centro per le Tecnologie della Salute (Center for Health Technologies – CHT), UdR INSTM, Università di Pavia, viale Taramelli 3/b, 27100 Pavia, Italy;Dipartimento di Scienze del Farmaco, Università di Pavia, viale Taramelli 12, 27100 Pavia, Italy;NABLA Lab, Biological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia;

关键词: silver nanoparticles;    fluoride;    antibacterial;    biofilm;    antibiofilm;    ionic silver;   

DOI  :  10.3390/molecules25153494

来源: DOAJ

【 摘 要 】

Silver nanoparticles were produced with AgF as the starting Ag(I) salt, with pectin as the reductant and protecting agent. While the obtained nanoparticles (pAgNP-F) have the same dimensional and physicochemical properties as those already described by us and obtained from AgNO₃ and pectin (pAgNP-N), the silver nanoparticles from AgF display an increased antibacterial activity against E. coli PHL628 and Staphylococcus epidermidis RP62A (S. epidermidis RP62A), both as planktonic strains and as their biofilms with respect to pAgNP-N. In particular, a comparison of the antimicrobial and antibiofilm action of pAgNP-F has been carried out with pAgNP-N, pAgNP-N and added NaF, pure AgNO₃, pure AgF, AgNO₃ and added NaF and pure NaNO₃ and NaF salts. By also measuring the concentration of the Ag⁺ cation released by pAgNP-F and pAgNP-N, we were able to unravel the separate contributions of each potential antibacterial agent, observing an evident synergy between p-AgNP and the F^- anion: the F^- anion increases the antibacterial power of the p-AgNP solutions even when F⁻ is just 10 µM, a concentration at which F^- alone (i.e., as its Na⁺ salt) is completely ineffective.

【 授权许可】

Unknown