Antibacterial and antibiofilm activity of Abroma augusta stabilized silver (Ag) nanoparticles against drug-resistant clinical pathogens

Frontiers in Molecular Biosciences

Molecular Biosciences

Faizan Abul Qais¹  Fohad Mabood Husain²  Amardeep Singh³  Mohammad Jalal⁴  Sachin Kumar⁴  Haris M. Khan⁴  Mo Ahamad Khan⁴  Uzma Tayyaba⁴  Syed Ghazanfar Ali⁴  Mohammad Shahid⁵  Byeong-Il Lee⁶  Rafiq Ahmad⁷ 

[1] Department of Ag. Microbiology, Faculty of Agriculture Sciences, Aligarh Muslim University, Aligarh, India;Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia;Department of Genetics and Plant Breeding, Chaudhary Charan Singh University, Meerut, Uttar Pradesh, India;Department of Microbiology, J. N. Medical College and Hospital, Aligarh Muslim University, Aligarh, Uttar Pradesh, India;Department of Microbiology, J. N. Medical College and Hospital, Aligarh Muslim University, Aligarh, Uttar Pradesh, India;Department of Microbiology, Immunology and Infectious Diseases, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain;Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan, Republic of Korea;Digital Healthcare Research Center, Institute of Information Technology and Convergence, Pukyong National University, Busan, Republic of Korea;Division of Smart Healthcare, College of Information Technology and Convergence, Pukyong National University, Busan, Republic of Korea;‘New-senior’ Oriented Smart Health Care Education Center, Pukyong National University, Busan, Republic of Korea;

关键词: silver nanoparticles;    green synthesis;    antibacterial;    Abroma augusta;    MRSA;    VRE;    biofilm;   

DOI  :  10.3389/fmolb.2023.1292509

 received in 2023-09-11, accepted in 2023-10-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

Infectious diseases remain among the most pressing concerns for human health. This issue has grown even more complex with the emergence of multidrug-resistant (MDR) bacteria. To address bacterial infections, nanoparticles have emerged as a promising avenue, offering the potential to target bacteria at multiple levels and effectively eliminate them. In this study, silver nanoparticles (AA-AgNPs) were synthesized using the leaf extract of a medicinal plant, Abroma augusta. The synthesis method is straightforward, safe, cost-effective, and environment friendly, utilizing the leaf extract of this Ayurvedic herb. The UV-vis absorbance peak at 424 nm indicated the formation of AA-AgNPs, with the involvement of numerous functional groups in the synthesis and stabilization of the particles. AA-AgNPs exhibited robust antibacterial and antibiofilm activities against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE). The MIC values of AA-AgNPs ranged from 8 to 32 μg/mL. Electron microscopic examination of the interaction of AA-AgNPs with the test bacterial pathogens showed a deleterious impact on bacterial morphology, resulting from membrane rupture and leakage of intracellular components. AA-AgNPs also demonstrated a dose-dependent effect in curtailing biofilm formation below inhibitory doses. Overall, this study highlights the potential of AA-AgNPs in the successful inhibition of both the growth and biofilms of MRSA and VRE bacteria. Following studies on toxicity and dose optimization, such AgNPs could be developed into effective medical remedies against infections.

【 授权许可】

Unknown   
Copyright © 2023 Kumar, Khan, Husain, Ahmad, Qais, Khan, Jalal, Tayyaba, Ali, Singh, Shahid and Lee.