Isolation of tumor-resident CD8+ T cells from human lung tumors

STAR Protocols

Yann Lecluse¹  Fathia Mami-chouaib²  Stéphanie Corgnac³ 

[1] Corresponding author;CNRS ums3655 INSERM us23 Gustave Roussy Cancer Campus, Villejuif 94805, France;INSERM UMR 1186, Integrative Tumor Immunology and Immunotherapy, Gustave Roussy, Fac. de Médecine - Univ. Paris-Sud, Université Paris-Saclay, Villejuif 94805, France;

关键词: Cell isolation;    Flow cytometry/mass cytometry;    Cancer;    Immunology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: CD103+CD8+ tumor-resident memory T cells (TRM) are important components of anti-tumor immunity. However, their role in response to cancer immunotherapy is not fully understood. The protocol describes how to isolate CD8+ T cells and autologous tumor cells from human lung tumors to study the functional activities of CD8+ T cells. Tumors are heterogeneous in terms of the quantity and quality of immune cell types, so the yield of TRM cells depends on the features of the tumor.For complete details on the use and execution of this protocol, please refer to Corgnac et al. (2020).

【 授权许可】

Unknown