Murine cochlear cell sorting and cell-type-specific organoid culture

STAR Protocols

Stefan Heller¹  Marie Kubota² 

[1] Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA;;Department of Otolaryngology – Head &

关键词: Cell Biology;    Cell culture;    Cell isolation;    Flow cytometry/mass cytometry;    Neuroscience;    Stem cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Neonatal mouse cochlear duct cells can proliferate and grow in vitro into inner ear organoids. Distinctive cochlear duct cell types have different organoid formation capacities. Here, we provide a flow cytometric cell-sorting method that allows the subsequent culture of individual cochlear cell populations. For the efficient culture of the sorted cells, we provide protocols for growing free-floating inner ear organoids, the adherence of organoids to a substrate, and the expansion of organoid-derived inner ear colonies.For complete details on the use and execution of this protocol, please refer to Kubota et al. (2021).

【 授权许可】

Unknown