Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment

STAR Protocols

Malay Haldar¹  Samir Devalaraja² 

[1] Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA;Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA;

关键词: Cell isolation;    Flow Cytometry/Mass Cytometry;    Cancer;    Immunology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The regulation of monocyte differentiation in the tumor microenvironment is of significant interest to tumor immunologists. Monocytes injected into the circulation may not track into tumors in sufficient numbers, making intratumoral injections a preferred experimental approach. Monocyte enrichment with antibody-based positive selection may activate downstream signaling, while cell sorters expose monocytes to mechanical stress. Here, we describe an approach of intratumoral monocyte transfer that circumvents these limitations by using negative selection and fluorescent reporter mice.For complete details on the use and execution of this protocol, please refer to Devalaraja et al. (2020).

【 授权许可】

Unknown