Acquisition, processing, and single-cell analysis of normal human breast tissues from a biobank

STAR Protocols

Natascia Marino¹  Harikrishna Nakshatri²  Anna Maria Storniolo³  Yunlong Liu³  Hongyu Gao⁴  Poornima Bhat-Nakshatri⁵ 

[1] Corresponding author;Indiana University Melvin and Bren Simon Comprehensive Cancer Center, Indiana University School of Medicine, Indianapolis, IN 46202, USA;Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202, USA;Department of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA;Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA;

关键词: Sequence analysis;    Cell Biology;    Single Cell;    Cancer;    Health Sciences;    Genomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The Komen Tissue Bank is the only biorepository in the world for normal breast tissues from women. Below we report the acquisition and processing of breast tissue from volunteer donors and describe an experimental and analysis pipeline to generate a single-cell atlas. This atlas is based on single-cell RNA-seq and is useful to derive breast epithelial cell subcluster-specific gene expression signatures, which can be applied to breast cancer gene expression data to identify putative cell-of-origin.For complete details on the use and execution of this protocol, please refer to Bhat-Nakshatri et al. (2021).

【 授权许可】

Unknown