scGR-seq: Integrated analysis of glycan and RNA in single cells

STAR Protocols

Haruka Ozaki¹  Haruki Odaka²  Hiroaki Tateno² 

[1] Bioinformatics Laboratory, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan;Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan;

关键词: Cell Biology;    Cell culture;    Single Cell;    Genomics;    Sequencing;    RNAseq;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Glycans are structurally diverse molecules found on the surface of living cells. The protocol details a system developed for combined analysis of glycan and RNA in single cells (scGR-seq) using human induced pluripotent stem cells (hiPSCs) and hiPSC-derived neural progenitor cells (NPCs). scGR-seq consists of DNA-barcoded lectin-based glycan profiling by sequencing (scGlycan-seq) and single-cell transcriptome profiling (scRNA-seq). scGR-seq will be an essential technique to delineate the cellular heterogeneity of glycans across multicellular systems.For complete details on the use and execution of this profile, please refer to Minoshima et al. (2021).

【 授权许可】

Unknown