A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome

STAR Protocols

Simon M. Loveday¹  Peter H. Janssen²  Karl Fraser³  Laura G. Raymond⁴  Stefan J. Hill⁵  Nicole C. Roy⁶  Ian M. Sims⁷  Warren C. McNabb⁷  Laura Payling⁸ 

[1] Corresponding author;Department of Nutrition, University of Otago, Dunedin 9016, New Zealand;Food and Bio-Based Products, AgResearch Limited, Tennent Drive, Palmerston North 4410, New Zealand;High-Value Nutrition National Science Challenge, Auckland 1023, New Zealand;Liggins Institute, The University of Auckland, Auckland 1023, New Zealand;Riddet Institute, Massey University, Palmerston North 4410, New Zealand;Riddet Institute, Massey University, Palmerston North 4410, New Zealand;School of Food and Advanced Technology, Massey University, Palmerston North 4410, New Zealand;

关键词: Cell Culture;    Clinical Protocol;    Genomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which combines standardized methods in human breath testing and fecal sampling. The model described is accessible and easy to repeat, but its relative simplicity means that it can deviate from human physiological conditions.

【 授权许可】

Unknown