Processing human urine and ureteral stents for 16S rRNA amplicon sequencing

STAR Protocols

Jeremy P. Burton¹  Kait F. Al² 

[1] Department of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada;Centre for Human Microbiome and Probiotic Research, Lawson Health Research Institute, London, ON, Canada;

关键词: Sequence analysis;    Clinical Protocol;    Genomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Ureteral stents are commonly used medical devices that harbor a unique and patient-specific microbial community. This protocol describes an optimized procedure for high-quality DNA extraction from both urine and ureteral stent samples for the purpose of downstream microbiota characterization by amplicon sequencing. Detailed instruction is provided for 16S rRNA gene V4 region sequencing with the Illumina platform, which enables accurate and reproducible microbiota profiling of low bacterial abundance urine and stent samples.For complete details on the use and execution of this protocol, please refer to Al et al. (2020).

【 授权许可】

Unknown