A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

STAR Protocols

Yoram Gerchman¹  Benjamin Trabelcy²  Amir Sapir² 

[1] Corresponding author;Faculty of Natural Sciences, University of Haifa, Haifa 3498838 Israel;

关键词: Genetics;    Mass Spectrometry;    Metabolism;    Model Organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol.For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).

【 授权许可】

Unknown