Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles

STAR Protocols

Edward Owusu-Ansah¹  Anjaneyulu Murari² 

[1] Corresponding author;Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, NY 10032, USA;

关键词: Cell Biology;    Genetics;    Metabolism;    Model Organisms;    Protein Biochemistry;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Drosophila flight muscles are highly enriched with mitochondria and have emerged as a powerful genetic system for studying how oxidative phosphorylation (OXPHOS) complexes are assembled. Here, we describe a series of protocols for analyzing the integrity of OXPHOS complexes in Drosophila via blue native polyacrylamide gel electrophoresis (BN PAGE). We have also included protocols for the additional steps that are typically performed after OXPHOS complexes are separated by BN PAGE, such as Coomassie staining, silver staining, and in-gel OXPHOS activities.For complete details on the use and execution of this protocol, please refer to Murari et al. (2020).

【 授权许可】

Unknown