A protocol for studying glucose homeostasis and islet function in mice

STAR Protocols

Michael B. Wheeler¹  Dana Al Rijjal² 

[1] Corresponding author;Department of Physiology, University of Toronto, 1 King's College Circle, Medical Science Building Rm3352, Toronto, ON M5S 1A8, Canada;

关键词: Cell Biology;    Cell isolation;    Metabolism;    Model Organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Glucose tolerance test and glucose stimulated insulin secretion are important measures to determine glucose homeostasis and islet function and assess diabetes. Here, we provide a protocol where glucose tolerance test is used to study glucose homeostasis and insulin secretion in vivo, followed by islet isolation and glucose stimulated insulin secretion to determine islet function ex vivo. This protocol enables evaluation of glucose homeostasis and islets in mice which can also be applied to rat, beta cell lines and human studies.For complete details on the use and execution of this profile, please refer to Al Rijjal et al. (2021).

【 授权许可】

Unknown