Determining the Cytotoxicity of Oxidized Lipids in Cultured Caco-2 Cells Using Bioimaging Techniques

Molecules

WafaS. Alansari¹  AreejA. Eskandrani²  Rabia Alghazeer³  Ghalia Shamlan⁴  AbdullahA. Burwaiss⁵  NazlinK. Howell⁶ 

[1] Biochemistry Department, Faculty of Science, University of Jeddah, Jeddah 21577, Saudi Arabia;Chemistry Department, Faculty of Science, Taibah University, Medina 30002, Saudi Arabia;Chemistry Department, Faculty of Science, University of Tripoli, Tripoli 50676, Libya;Department of Food Science and Nutrition, College of Food and agriculture Sciences, King Saud University, Riyadh 11362, Saudi Arabia;Medicine Department, Faculty of Human Medicine, University of Tripoli, Tripoli 50676, Libya;School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK;

关键词: fish oil;    lipid peroxidation;    ESR spectroscopy;    AFM;    Raman microscopy;   

DOI  :  10.3390/molecules25071693

来源: DOAJ

【 摘 要 】

Fish lipids are comprised of considerable quantities of polyunsaturated acids and are prone to oxidation, producing reactive oxygen species and hydroperoxides. This study aimed to evaluate the biochemical and structural alterations in Caco-2 cells following exposure to 100 μg/mL methyl linoleate or fish oil, and then radiated for 24, 48 or 72 h. Electron spin resonance spectroscopy detected free radicals in the lipid membrane, Raman microscopy observed biochemical alterations and atomic force microscopy identified changes in morphology, such as the breakdown of DNA bonds. The study showed that bioimaging and biochemical techniques can be effective at detecting and diagnosing cellular injuries incurred by lipid peroxidation.

【 授权许可】

Unknown