Survey on Carbapenem-Resistant Bacteria in Pigs at Slaughter and Comparison with Human Clinical Isolates in Italy

Antibiotics

Martina Rega¹  Sandro Cavirani¹  Costanza Spadini¹  Clotilde Silvia Cabassi¹  Cristina Bacci¹  Mattia Iannarelli¹  Silvia Bonardi¹  Gerardo Manfreda²  Frédérique Pasquali²  Cecilia Crippa²  Antonio Parisi³  Ferdinando Ruocco⁴  Alice Sabatino⁵  Enrico Fiaccadori⁵ 

[1] Department of Veterinary Science, University of Parma, 43126 Parma, Italy;Food Safety Unit, Department of Agricultural and Food Sciences Alma Mater Studiorum, University of Bologna, 40064 Ozzano dell’Emilia, Italy;Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 70017 Putignano, Italy;National Veterinary Service, 43126 Parma, Italy;Nephrology Unit, Parma University-Hospital, Department of Medicine and Surgery, Parma University, 43126 Parma, Italy;

关键词: carbapenem resistance;    ESBLs;    OXA genes;    antimicrobial resistance;    Pseudomonas aeruginosa;    pigs;   

DOI  :  10.3390/antibiotics11060777

来源: DOAJ

【 摘 要 】

This study is focused on resistance to carbapenems and third-generation cephalosporins in Gram-negative microorganisms isolated from swine, whose transmission to humans via pork consumption cannot be excluded. In addition, the common carriage of carbapenem-resistant (CR) bacteria between humans and pigs was evaluated. Sampling involved 300 faecal samples collected from slaughtered pigs and 300 urine samples collected from 187 hospitalised patients in Parma Province (Italy). In swine, MIC testing confirmed resistance to meropenem for isolates of Pseudomonas aeruginosa and Pseudomonas oryzihabitans and resistance to cefotaxime and ceftazidime for Escherichia coli, Ewingella americana, Enterobacter agglomerans, and Citrobacter freundii. For Acinetobacter lwoffii, Aeromonas hydrofila, Burkolderia cepacia, Corynebacterium indologenes, Flavobacterium odoratum, and Stenotrophomonas maltophilia, no EUCAST MIC breakpoints were available. However, ESBL genes (bla_CTXM-1, bla_CTX-M-2, bla_TEM-1, and bla_SHV) and AmpC genes (bla_CIT, bla_ACC, and bla_EBC) were found in 38 and 16 isolates, respectively. P. aeruginosa was the only CR species shared by pigs (4/300 pigs; 1.3%) and patients (2/187; 1.1%). P. aeruginosa ST938 carrying bla_PAO and bla_OXA396 was detected in one pig as well as an 83-year-old patient. Although no direct epidemiological link was demonstrable, SNP calling and cgMLST showed a genetic relationship of the isolates (86 SNPs and 661 allele difference), thus suggesting possible circulation of CR bacteria between swine and humans.

【 授权许可】

Unknown