Dp71 depleted HBE cells displayed increased DNA damage and apoptosis induced by H2O2

Cellular & Molecular Biology Letters

Jinliang Xie¹  Xuefei Xiao²  Sichuang Tan³  Shuai Zhao³  Lan Xiao⁴  Sipin Tan⁵ 

[1] Center of Transplant Surgery, Xiangya Hospital, Central South University;Department of Emergency and Critical Care Medicine, the Third Xiangya Hospital, Central South University;Department of Thoracic Surgery, the Second Xiangya Hospital, Central South University;Department of Traditional Chinese Medicine, the Third Xiangya Hospital, Central South University;Key Laboratory of Sepsis Translational Medicine of Hunan, Department of Pathophysiology, Xiangya School of Medicine, Central South University;

关键词: Dp71;    DNA damage;    Apoptosis;    RAD51;    FAK;   

DOI  :  10.1186/s11658-019-0169-6

来源: DOAJ

【 摘 要 】

Abstract Human bronchial epithelium (HBE)-Dp71 anti-sense(AS)cells with stably transfected Dp71 siRNA plasmids were prepared for further exploration of Dp71 biological traits in cells other than PC12. HBE-Dp71AS cells displayed increased DNA damage induced by H2O2. Apoptosis of HBE-Dp71AS cells induced by H2O2 was increased via enhancing caspase 3, caspase 8 and caspase 9. HBE-Dp71AS cells also displayed decreased proliferation and clonogenic formation. RAD51 was proved to be a new binding partner of Dp71 by co-immunoprecipitation (Ip) and immunofluorescence. Reduced RAD51 mRNA and protein levels were observed in HBE-Dp71AS cells. Decreased lamin B1, focal adhesion kinase (FAK), phosphorylated focal adhesion kinase (p-FAK) and phosphorylated protein kinase B (p-AKT) were detected in the HBE-Dp71AS cells, which functioned together with RAD51 as the molecular explanations for the character alterations of HBE-Dp71AS cells.

【 授权许可】

Unknown