The ruthenium complex cis-(dichloro)tetrammineruthenium(III) chloride induces apoptosis and damages DNA in murine sarcoma 180 cells

Journal of biosciences

Luiz Alfredo Pavanin²  Wagner Batista Dos Santos³  Cesar Augusto Sam Tiago Vilanova-Costa¹  Alessandra De Santana Braga Barbosa Ribeiro¹  Flávia De Castro Pereira¹  Aliny Pereira De Lima¹  Elisângela De Paula Silveira-Lacerda1¹ 

[1] Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas, Universidade Federal de Goiás - UFG, Goiânia, Goiás, Brazil$$;Instituto de Química, Universidade Federal de Uberlândia - UFU, Uberlândia, Minas Gerais, Brazil$$;Faculdade Patos de Minas - FPM, Patos de Minas, Minas Gerais, Brazil$$

关键词: Apoptosis;    cis-(dichloro)tetrammineruthenium(III) chloride;    cell cycle;    DNA damage;    S-180;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Ruthenium(III) complexes are increasingly attracting the interest of researchers due to their promising pharmacological properties. Recently, we reported that the cis-(dichloro)tetrammineruthenium(III) chloride compound has cytotoxic effects on murine sarcoma 180 (S-180) cells. In an effort to understand the mechanism responsible for their cytotoxicity, study we investigated the genotoxicity, cell cycle distribution and induction of apoptosis caused by cis-(dichloro)tetrammineruthenium(III) chloride in S-180 tumour cells. cis-(dichloro)tetrammineruthenium(III) chloride treatment induced significant DNA damage in S-180 cells, as detected by the alkaline comet assay. In the cell cycle analysis, cis-(dichloro)tetrammineruthenium(III) chloride caused an increase in the number of cells in G1 phase, accompanied by a decrease in the S and G2 phases after 24 h of treatment. In contrast, the cell cycle distribution of S-180 cells treated with cis-(dichloro)tetrammineruthenium(III) chloride for 48 h showed a concentration-dependent increase in the sub-G1 phase (indicating apoptosis), with a corresponding decrease in cells in the G1, S and G2 phases. In addition, cis-(dichloro)tetrammineruthenium(III) chloride treatment induced apoptosis in a time-dependent manner, as observed by the increased numbers of annexin V-positive cells. Taken together, these findings strongly demonstrate that DNA damage, cell cycle changes and apoptosis may correlate with the cytotoxic effects of cis-(dichloro)tetrammineruthenium(III) chloride on S-180 cells.

【 授权许可】

Unknown