Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide

International Journal of Environmental Research and Public Health

Onyinye Nwagbara¹  Selina F. Darling-Reed³  Alicia Tucker³  Cynthia Harris²  Michael Abazinge¹  Ronald D. Thomas³ 

[1] Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA;Institute of Public Health, Florida A&M University, Tallahassee, FL 32307, USA;College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA

关键词: Benzo[a]pyrene;    benzo[a]pyrene-7;    8-diol-9;    10-epoxide;    DU145;    cell viability;    DNA damage;    cell cycle;   

DOI  :  10.3390/ijerph2007010002

来源: mdpi

【 摘 要 】

Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a major environmental pollutant. In this study, the effects of this carcinogen/mutagen and one of its metabolites, benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), on human prostate carcinoma cell line DU145, were examined. Cell viability, DNA damage, and cell cycle progression were evaluated as toxic end-points. We have shown that B[a]P and BPDE inhibited cell viability following 48 hr of exposure. Furthermore, comet assay analyses revealed that both B[a]P and BPDE induced DNA strand breaks in a concentration -dependent fashion. Flow cytometric analyses showed that about 70 % of DU145 cells were arrested by B[a]P at the G₁ phase, while about 76% were arrested at G₁ phase by BPDE. These data reveal that B[a]P and BPDE are cytotoxic and genotoxic to DU145 prostate cancer cells.

【 授权许可】

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