Alpha-Ketoglutaric Acid Production from a Mixture of Glycerol and Rapeseed Oil by Yarrowia lipolytica Using Different Substrate Feeding Strategies

Sustainability

Ludwika Tomaszewska-Hetman¹  Anita Rywińska¹  Magdalena Rakicka-Pustułka¹  Piotr Juszczyk¹  Waldemar Rymowicz¹ 

[1] Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego St. 37, 51-630 Wrocław, Poland;

关键词: Yarrowia lipolytica;    α-ketoglutaric acid;    glycerol;    rapeseed oil;    substrate feeding strategies;    fed-batch culture;   

DOI  :  10.3390/su12156109

来源: DOAJ

【 摘 要 】

The microbiological biosynthesis of α-ketoglutaric acid (KGA) has recently captured the attention of many scientists as an alternative to its common chemical synthesis. The present study aimed to evaluate the effect of the feeding strategy of substrates, i.e., glycerol (G = 20 g·dm⁻³) and rapeseed oil (O = 20 g·dm⁻³), on yeast growth and the parameters of KGA biosynthesis by a wild strain Yarrowia lipolytica A-8 in fed-batch and repeated-batch cultures. The effectiveness of KGA biosynthesis was demonstrated to depend on thiamine concentration and the substrate feeding method. In the fed-batch culture incubated with 3 µg·dm⁻³ of thiamine and a substrate feeding variant 2G(_OGO), KGA was produced in the amount of 62.1 g·dm⁻³ at the volumetric production rate of 0.37 g·dm⁻³·h⁻¹. These values of KGA production parameters were higher than these obtained in the control culture (with rapeseed oil only). During 10 cycles of the 1788-h repeated-batch culture carried out acc. to the feeding strategy 2G(_OGO), in the last 5 cycles the yeast produced from 55.6 to 58.2 g·dm⁻³ of KGA and maximally 2.9 g·dm⁻³ of the pyruvic acid as a by-product.

【 授权许可】

Unknown