Application of a New Engineered Strain of Yarrowia lipolytica for Effective Production of Calcium Ketoglutarate Dietary Supplements

International Journal of Molecular Sciences

卷:22

Tomasz Janek¹  Ludwika Tomaszewska-Hetman¹  Marta Kuźmińska-Bajor¹  Anita Rywińska¹  Magdalena Rakicka-Pustułka¹  Piotr Juszczyk¹  Zbigniew Lazar¹  Waldemar Rymowicz¹ 

[1] Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, Poland;

关键词: Yarrowia lipolytica;    α-ketoglutaric acid (KGA);    calcium ketoglutarate;    dietary supplement;    metabolic engineering;    glycerol;   

DOI  :  10.3390/ijms22147577

来源: DOAJ

【 摘 要 】

The present study aimed to develop a technology for the production of dietary supplements based on yeast biomass and α-ketoglutaric acid (KGA), produced by a new transformant of Yarrowia lipolytica with improved KGA biosynthesis ability, as well to verify the usefulness of the obtained products for food and feed purposes. Transformants of Y. lipolytica were constructed to overexpress genes encoding glycerol kinase, methylcitrate synthase and mitochondrial organic acid transporter. The strains were compared in terms of growth ability in glycerol- and oil-based media as well as their suitability for KGA biosynthesis in mixed glycerol–oil medium. The impact of different C:N:P ratios on KGA production by selected strain was also evaluated. Application of the strain that overexpressed all three genes in the culture with a C:N:P ratio of 87:5:1 allowed us to obtain 53.1 g/L of KGA with productivity of 0.35 g/Lh and yield of 0.53 g/g. Finally, the possibility of obtaining three different products with desired nutritional and health-beneficial characteristics was demonstrated: (1) calcium α-ketoglutarate (CaKGA) with purity of 89.9% obtained by precipitation of KGA with CaCO₃, (2) yeast biomass with very good nutritional properties, (3) fixed biomass-CaKGA preparation containing 87.2 μg/g of kynurenic acid, which increases the health-promoting value of the product.

【 授权许可】

Unknown