Biotechnological Potential of Yarrowia lipolytica Grown under Thiamine Limitation

Food Technology and Biotechnology

Alsu I. Yusupova¹  Valentina Ya. Lysanskaya¹  Natalia G. Vinokurova¹  Svetlana V. Kamzolova¹  Igor G. Morgunov¹  Maria N. Chiglintseva² 

[1] G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia;Pushchino Branch of Moscow State University, Microdistrict 'V' 25, Pushchino, RU-142290 Moscow Region, Russia;

关键词: microbial production;    succinic acid;    α-ketoglutaric acid;    Yarrowia lipolytica;    γ-aminobutyric acid;    diethyl succinate;   

DOI  :  

来源: DOAJ

【 摘 要 】

During the cultivation of a thiamine-auxotrophic yeast strain Yarrowia lipolytica VKM Y-2412 on ethanol, the growth limitation by thiamine leads to the production of α-ketoglutaric acid. The α-ketoglutaric acid synthesis has been studied in dependence on pH, oxygen supply and ethanol, zinc and iron concentrations. Under optimal conditions, Y. lipolytica produced 88.7 g/L of α-ketoglutaric acid. The culture broth containing α-ketoglutaric acid was subjected to chemical treatment with hydrogen peroxide, which led to the formation of succinic acid in significant quantities (71.7 g/L). Further direct esterification of succinic acid with excess absolute ethanol yielded diethyl succinate. Biomass of Y. lipolytica, a superproducer of α-ketoglutaric acid, was characterized by a high content of protein and essential amino acids, free amino acids, and unusually large amount of γ-aminobutyric acid. The unique amino acid composition of the producer makes it possible to use this biomass as a component of parenteral nutrition mixtures and as a basis for neuroleptics.

【 授权许可】

Unknown