Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions—Ara h2 and Ara h6 Are Readily Bio-Accessible

Nutrients

Joe Baumert¹  RobertJan Veldman²  Mieke Smits²  Harry Wichers²  GovardusA.H. de Jong³  StefJ. Koppelman⁴  Renger Witkamp⁴  Raymond Pieters⁴  Monic Tomassen⁵  SteveL. Taylor⁶ 

[1] Biobased Research, Wageningen University and Research, Bornse Weilanden 9, P.O. Box 17, 6700 AA Wageningen, The Netherlands;Technology, University of Nebraska, 279 Food Innovation Center, Lincoln, NE 68588-6207, USA;;Food &;Food Allergy Research and Resource Program, Department of Food Science &Research Group Innovative Testing in Life Sciences and Chemistry, University of Applied Sciences, Heidelberglaan 7, 3584 CS Utrecht, The Netherlands;TNO, Utrechtseweg 48, 3704 HE Zeist, The Netherlands;

关键词: peanut;    Arachis hypogaea;    allergen;    bio-accessibility;    saliva;   

DOI  :  10.3390/nu10091281

来源: DOAJ

【 摘 要 】

The oral mucosa is the first immune tissue that encounters allergens upon ingestion of food. We hypothesized that the bio-accessibility of allergens at this stage may be a key determinant for sensitization. Light roasted peanut flour was suspended at various pH in buffers mimicking saliva. Protein concentrations and allergens profiles were determined in the supernatants. Peanut protein solubility was poor in the pH range between 3 and 6, while at a low pH (1.5) and at moderately high pHs (>8), it increased. In the pH range of saliva, between 6.5 and 8.5, the allergens Ara h2 and Ara h6 were readily released, whereas Ara h1 and Ara h3 were poorly released. Increasing the pH from 6.5 to 8.5 slightly increased the release of Ara h1 and Ara h3, but the recovery remained low (approximately 20%) compared to that of Ara h2 and Ara h6 (approximately 100% and 65%, respectively). This remarkable difference in the extraction kinetics suggests that Ara h2 and Ara h6 are the first allergens an individual is exposed to upon ingestion of peanut-containing food. We conclude that the peanut allergens Ara h2 and Ara h6 are quickly bio-accessible in the mouth, potentially explaining their extraordinary allergenicity.

【 授权许可】

Unknown