Quantification and Improvement of the Dynamics of Human Serum Albumin and Glycated Human Serum Albumin with Astaxanthin/Astaxanthin-Metal Ion Complexes: Physico-Chemical and Computational Approaches

International Journal of Molecular Sciences

Syahputra Wibowo¹  Sutiman Bambang Sumitro¹  Sri Widyarti¹  Maria Camilla Baratto²  Jessica Costa²  Rebecca Pogni²  Akhmad Sabarudin³  Koichi Matsuo⁴ 

[1]Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia

[2]Department of Biotechnology, Chemistry and Pharmacy, Università di Siena, Via A. Moro 2, 53100 Siena, Italy

[3]Department of Chemistry, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia

[4]Hiroshima Synchrotron Radiation Center, Hiroshima University, Higashi-Hiroshima 739-0046, Japan

关键词: antioxidants;    astaxanthin;    astaxanthin metal complexes;    human serum albumin;    glycated human serum albumin;    diabetes mellitus type 2;   

DOI  :  10.3390/ijms23094771

来源: DOAJ

【 摘 要 】

Glycated human serum albumin (gHSA) undergoes conformational changes and unfolding events caused by free radicals. The glycation process results in a reduced ability of albumin to act as an endogenous scavenger and transporter protein in diabetes mellitus type 2 (T2DM) patients. Astaxanthin (ASX) in native form and complexed with metal ions (Cu²⁺ and Zn²⁺) has been shown to prevent gHSA from experiencing unfolding events. Furthermore, it improves protein stability of gHSA and human serum albumin (HSA) as it is shown through molecular dynamics studies. In this study, the ASX/ASX-metal ion complexes were reacted with both HSA/gHSA and analyzed with electronic paramagnetic resonance (EPR) spectroscopy, rheology and zeta sizer (particle size and zeta potential) analysis, circular dichroism (CD) spectroscopy and UV-Vis spectrophotometer measurements, as well as molecular electrostatic potential (MEP) and molecular docking calculations. The addition of metal ions to ASX improves its ability to act as an antioxidant and both ASX or ASX-metal ion complexes maintain HSA and gHSA stability while performing their functions.

【 授权许可】

Unknown