Characterization and drug sensitivity of a novel human ovarian clear cell carcinoma cell line genomically and phenotypically similar to the original tumor

Cancer Medicine

Nick Bown¹  John Lunec²  Maryam Zanjirband²  Rachel O'Donnell²  Lucy Gentles²  Elizabeth Matheson²  Connor Gilkes‐Immeson²  Yvette Drew²  Miriam Franklin²  Alice Bradbury²  Nicola J. Curtin²  Paul Cross³  Angela Ralte³ 

[1] Northern Genetics Service Institute of Genetic Medicine Newcastle upon Tyne UK;Northern Institute for Cancer Research Medical School Newcastle University Newcastle upon Tyne UK;Pathology Department Queen Elizabeth Hospital Gateshead UK;

关键词: cytotoxic drugs;    DNA repair;    loss of heterozygosity;    new cell line;    NVP‐BEZ235;    ovarian clear cell carcinoma;   

DOI  :  10.1002/cam4.1724

来源: DOAJ

【 摘 要 】

Abstract NUCOLL43 is a novel ovarian clear cell carcinoma (O‐CCC) cell line that arose from a primary culture of a patient's malignant ascites. The cells grow reliably in cell culture with a doubling time of approx. 45 hours and form colonies at high efficiency. They have a very high degree of loss of heterozygosity (LOH) affecting approximately 85% of the genome, mostly copy neutral and almost identical to the original tumor. The cells express epithelial (pan‐cytokeratin) and mesenchymal (vimentin) characteristics, CA125 and p16, like the original tumor. They also express ARID1A but not HNF‐1β and, like the original tumor, and are negative for p53 expression, with no evidence of p53 function. NUCOLL43 cells express all other DNA damage response proteins investigated and have functional homologous recombination DNA repair. They are insensitive to cisplatin, the PARP inhibitor rucaparib, and MDM2 inhibitors but are sensitive to camptothecin, paclitaxel, and NVP‐BEZ235. The NUCOLL43 cell line represents a distinct subtype of O‐CCC that is p53 and HNF‐1β null but expresses ARID1A. Its high degree of similarity with the original tumor genomically and proteomically, as well as the high level of LOH, make this an interesting cell line for O‐CCC research. It has been deposited with Ximbio.

【 授权许可】

Unknown