Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus

Food Science and Technology

Maria Aparecida Fernandez¹  Wladimir Padilha da Silva²  Eliezer Avila Gandra²  Jorge Adolfo Silva² 

[1] Universidade Estadual de Maringá;Universidade Federal de Pelotas;

关键词: S. aureus;    S. hyicus;    S. intermedius;    PCR;    gene nuc;   

DOI  :  10.1590/S0101-20612011000400019

来源: DOAJ

【 摘 要 】

The enterotoxigenic species Staphylococcus aureus, S. hyicus and S. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. This study aimed at the development of a Multiplex PCR (mPCR) for the identification of S. aureus, S. intermedius and S. hyicus using the nuc gene as the target sequence. The results obtained suggest that the set of primers used was specific for the three species of Staphylococcus evaluate with a detection limit of 10² CFU.mL-1.

【 授权许可】

Unknown