Food Science and Technology (Campinas) | |
Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus | |
Eliezer Avila Gandra2  Maria Aparecida Fernandez1  Jorge Adolfo Silva1  Wladimir Padilha Da Silva1  | |
[1] ,Universidade Federal de Pelotas Centro de Ciências Químicas, Farmacêuticas e de Alimentos Pelotas RS ,Brasil | |
关键词: S. aureus; S. hyicus; S. intermedius; PCR; nuc gene; S. aureus; S. hyicus; S. intermedius; PCR; gene nuc; | |
DOI : 10.1590/S0101-20612011000400019 | |
来源: SciELO | |
【 摘 要 】
The enterotoxigenic species Staphylococcus aureus, S. hyicus and S. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. This study aimed at the development of a Multiplex PCR (mPCR) for the identification of S. aureus, S. intermedius and S. hyicus using the nuc gene as the target sequence. The results obtained suggest that the set of primers used was specific for the three species of Staphylococcus evaluate with a detection limit of 10² CFU.mL-1.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
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RO202005130080970ZK.pdf | 603KB | download |