NZ-GMP approved serum improve hDPSC osteogenic commitment and increase angiogenic factor expression

Frontiers in Physiology

Roberta Montella¹  Anna Spina²  Marcella La Noce³  Alfredo De Rosa³  Davide Liccardo³  Thimios Mitsiadis⁴  Luigi Laino⁵ 

[1] GSK Vaccines;Istituto Nazionale Tumori - IRCCS Fondazione G.Pascale;Second University of Naples;University of Zurich;Università degli Studi di Foggia;

关键词: Osteogenesis;    Angiogenesis;    Bioscaffold;    hDPSCs;    Bone differentiation;   

DOI  :  10.3389/fphys.2016.00354

来源: DOAJ

【 摘 要 】

Human dental pulp stem cells (hDPSCs), selected from the stromal-vascular fraction of dental pulp, are ecto-mesenchymal stem cells deriving from neural crests, successfully used in human bone tissue engineering. For their use in human therapy GMP procedures are required. For instance, the use of fetal bovine serum (FBS) is strongly discouraged in clinical practice due to its high risk of prions and other infections for human health. Alternatively, clinical grade sera have been suggested, including the New Zealand FBS (NZ-FBS). Therefore, the aim of this study was to evaluate the behavior of hDPSCs expanded in culture medium containing NZ-FBS. Since it was widely demonstrated hDPSCs display relevant capabilities to differentiate into osteogenic and angiogenic lineages, we performed a comparative study to assess if these features are also retained by cultivating the cells with a safer serum never tested on this cell line. hDPSCs were grown using NZ-FBS and conventional (C-FBS) for 7, 14 and 21 days, in both 2D and 3D cultures. Growth curves, expression of bone-related markers, calcification and angiogenesis were evaluated. NZ-FBS induced significant cell growth with respect to C-FBS and promoted an earlier increase expression of osteogenic markers, in particular of those involved in the formation of mineralized matrix (BSP and OPN) within 14 days. In addition, hDPSCs cultured in presence of NZ-FBS were found to produce higher mRNA levels of the angiogenic factors, such as VEGF and PDGFA. Taken together, our results highlight that hDPSCs proliferate, enhance their osteogenic commitment and increase angiogenic factors in NZ-FBS containing medium. These features have also been found when hDPSC were seeded on the clinical-grade collagen I scaffold (Bio-Gide®), leading to the conclusion that for human therapy some procedures and above all the use of GMP-approved materials have no negative impact.

【 授权许可】

Unknown