Shear-Stress-Gradient and Oxygen-Gradient Ektacytometry in Sickle Cell Patients at Steady State and during Vaso-Occlusive Crises

Cells

Yves Bertrand¹  Alexandra Gauthier²  Solène Poutrel²  Elie Nader²  Emeric Stauffer²  Philippe Joly²  Emilie Virot²  Céline Renoux²  Camille Boisson²  Philippe Connes²  Arnaud Hot³  Giovanna Cannas³  Romain Fort⁴ 

[1] Institut d’Hématologique et d’Oncologique Pédiatrique, Hospices Civils de Lyon, 69008 Lyon, France;Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM) EA7424, Team “Vascular Biology and Red Blood Cell”, Université Claude Bernard Lyon 1, 69008 Lyon, France;Service de Médecine Interne, Hôpital Edouard Herriot, Hospices Civils de Lyon, 69008 Lyon, France;Service de Réanimation Médicale, Hôpital Lyon Sud, Hospices Civils de Lyon, 69495 Pierre-Bénite, France;

关键词: sickle cell disease;    red blood cell deformability;    oxygen gradient ektacytometry;    vaso-occlusive crisis;   

DOI  :  10.3390/cells11030585

来源: DOAJ

【 摘 要 】

Oxygen gradient ektacytometry (oxygenscan) measures the changes in red blood cell (RBC) deformability in normoxia and during deoxygenation. We investigated the changes in RBC deformability, measured by both oxygenscan and classical shear-stress-gradient ektacytometry, in 10 patients with sickle cell disease (SCD) during vaso-occlusive crisis (VOC) versus steady state. Oxygenscan and shear-stress-gradient ektacytometry parameters were also measured in 38 SCD patients at steady state on two different occasions. Shear-stress-gradient ektacytometry parameters, maximal RBC deformability at normoxia and the minimum RBC deformability during deoxygenation were lower during VOC compared to steady state. The oxygen partial pressure at which RBCs started to sickle (PoS) was not significantly affected by VOC, but the results were very heterogeneous: the PoS increased in 5 in 10 patients and decreased in 4 in 10 patients. Both oxygenscan and shear-stress-gradient ektacytometry parameters remained unchanged in patients at steady state between two sets of measurements, performed at 17 ± 8 months intervals. In conclusion, the present study showed that both oxygen gradient ektacytometry and shear-stress-gradient ektacytometry are sensitive to disease activity in SCD, and that both techniques give comparable results; however, the oxygen-dependent propensity of RBCs to sickle was highly variable during VOC.

【 授权许可】

Unknown