TRACE: An Unbiased Method to Permanently Tag Transiently Activated Inputs

Frontiers in Cellular Neuroscience

Sadegh Nabavi¹  Marco Capogna¹  Meet Jariwala¹  Nathalie Krauth²  Valentina Khalil²  Noemie Mermet-Joret²  Anne-Katrine Vestergaard² 

[1] Center for Proteins in Memory—PROMEMO, Danish National Research Foundation, Aarhus, Denmark;DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark;Department of Biomedicine, Aarhus University, Aarhus, Denmark;Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark;

关键词: neural circuits;    optogenetics;    viral tracing;    innate fear;    mouse behavior;   

DOI  :  10.3389/fncel.2020.00114

来源: DOAJ

【 摘 要 】

A fundamental interest in circuit analysis is to parse out the synaptic inputs underlying a behavioral experience. Toward this aim, we have devised an unbiased strategy that specifically labels the afferent inputs that are activated by a defined stimulus in an activity-dependent manner. We validated this strategy in four brain circuits receiving known sensory inputs. This strategy, as demonstrated here, accurately identifies these inputs.

【 授权许可】

Unknown