Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex

eLife

Yasushi Nakagawa¹  Anna Hoerder-Suabedissen²  Filippo Ghezzi²  Daniel Lyngholm²  Alexandra Rowett²  Zoltan Molnar²  Cristiana Vagnoni²  Gokul Parameswaran²  Paul G Anastasiades²  Andre Marques-Smith²  Simon JB Butt² 

[1] Department of Neuroscience, University of Minnesota, Minneapolis, United States;Department of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United Kingdom;

关键词: neocortex;    neural development;    subplate;    neural circuits;    optogenetics;    laser scanning photostimulation;    Mouse;   

DOI  :  10.7554/eLife.60810

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatosensory cortex (S1BF). These SPNs comprise of two morphological subtypes: fusiform SPNs with local axons and pyramidal SPNs with axons that extend through the marginal zone. The former receive translaminar synaptic input up until the emergence of the whisker barrels, a timepoint coincident with significant cell death. In contrast, pyramidal SPNs receive local input from the subplate at early ages but then – during the later time window – acquire input from overlying cortex. Combined electrical and optogenetic activation of thalamic afferents identified that Lpar1-EGFP SPNs receive sparse thalamic innervation. These data reveal components of the postnatal network that interpret sparse thalamic input to direct the emergent columnar structure of S1BF.

【 授权许可】

CC BY