eLife | |
Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex | |
Yasushi Nakagawa1  Anna Hoerder-Suabedissen2  Filippo Ghezzi2  Daniel Lyngholm2  Alexandra Rowett2  Zoltan Molnar2  Cristiana Vagnoni2  Gokul Parameswaran2  Paul G Anastasiades2  Andre Marques-Smith2  Simon JB Butt2  | |
[1] Department of Neuroscience, University of Minnesota, Minneapolis, United States;Department of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United Kingdom; | |
关键词: neocortex; neural development; subplate; neural circuits; optogenetics; laser scanning photostimulation; Mouse; | |
DOI : 10.7554/eLife.60810 | |
来源: eLife Sciences Publications, Ltd | |
【 摘 要 】
Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatosensory cortex (S1BF). These SPNs comprise of two morphological subtypes: fusiform SPNs with local axons and pyramidal SPNs with axons that extend through the marginal zone. The former receive translaminar synaptic input up until the emergence of the whisker barrels, a timepoint coincident with significant cell death. In contrast, pyramidal SPNs receive local input from the subplate at early ages but then – during the later time window – acquire input from overlying cortex. Combined electrical and optogenetic activation of thalamic afferents identified that Lpar1-EGFP SPNs receive sparse thalamic innervation. These data reveal components of the postnatal network that interpret sparse thalamic input to direct the emergent columnar structure of S1BF.
【 授权许可】
CC BY
【 预 览 】
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