Improved detection of esp, hyl, asa1, gelE, cylA virulence genes among clinical isolates of Enterococci

BMC Research Notes

Praveen Shabana¹  Alexander Kiruthiga²  Kesavaram Padmavathy²  Jeevan Malaiyan³  Sumathi Gnanadesikan³  Venkatesan Naveenkumar⁴ 

[1] Department of Laboratory Medicine, Meitra Hospital;Department of Microbiology, Research Laboratory for Oral and Systemic Health, Sree Balaji Dental College and Hospital, BIHER;Department of Microbiology, Sri Muthukumaran Medical College Hospital and Research Institute;ImmuGenix Biosciences Pvt Ltd;

关键词: E. faecalis;    E. faecium;    Virulence;    Modified PCR reactions;   

DOI  :  10.1186/s13104-020-05018-0

来源: DOAJ

【 摘 要 】

Abstract Objective Virulence factors (VFs) among the clinical strains of enterococci play a vital role in pathogenesis. This study was aimed to screen for cylA, asa1, gelE, esp and hyl among Enterococcus faecalis (n = 89) and E. faecium (n = 51) by multiplex PCR. The previously reported multiplex PCR was modified to 2 duplex (asa1 and gelE, cylA and esp) PCRs and 1 simplex (hyl) PCR. The idea of the modification of the multiplex PCR proposed here emerged in the course of the research study when majority of the isolates which phenotypically exhibited virulence traits were found to be negative for the respective gene. Results cylA, gelE and asa1 were significantly predominant in E. faecalis (59.55%, 85.39%, 86.51%) than E. faecium (1.96%, 60.78%, 9.80%) (p < 0.0001, p = 0.001967, p < 0.0001). hyl was detected in E. faecium (5.9%) only. The number of VFs detected in each isolate was recorded as the VF score. E. faecalis isolates had a VF score pattern of score 4 (34.83%), score 3 (26.96%), score 2 (28.08%) and score 1 (8.98%) while E. faecium had score 4 (1.96%), score 3 (7.84%), score 2 (25.49%) and score 1 (41.18%). This modification of the PCR protocol could resolve the problem of decreased detection of virulence determinants in enterococci.

【 授权许可】

Unknown