CBM.DIAGB.03.10.LLNL.007 Final Report

Slezak, T ; Torres, M

Lawrence Livermore National Laboratory

关键词: Vectors;    Genes;    59 Basic Biological Sciences;    Genetic Engineering;    Virulence;   

DOI  :  10.2172/1022152
RP-ID  :  LLNL-TR-478794
RP-ID  :  W-7405-ENG-48
RP-ID  :  1022152

美国|英语

来源: UNT Digital Library

【 摘 要 】

The purpose of this project was to construct a system for characterizing the threat potential of genomic sequences, specifically assembled draft genomes. New genomes are characterized by initially comparing them against already-sequenced genomes. If the new genome is determined to be from a high-threat species, detailed (forensic-level) characterization is done based on gene and SNP (Single Nucleotide Polymorphism) data comparisons with all other previously sequenced members of that high-threat species. New genomes are compared against a large set of known virulence and antibiotic-resistance genes and also compared against a large set of vectors that could be used for bacterial genetic engineering. Together, these analyses provide a comprehensive initial assessment of the most likely phylogenetic placement of a new genome, plus an assessment of the known-gene content and an indication of any possible bacterial genetic engineering utilizing vector-mediated techniques. This provides an initial threat potential summary based on high information content comparisons (e.g., thousands of genes, SNPs, and potential genetic engineering vectors) that can be used to guide subsequent operational response or more detailed laboratory characterization.