A New Selectable Marker System for Genetic Studies of Bacteria: Final Report

Parsons, D ; Tolmasky, M ; Chain, P ; Segelke, B W

Lawrence Livermore National Laboratory

关键词: Genetics;    Genes;    59 Basic Biological Sciences;    Genetic Engineering;    Mutagenesis;   

DOI  :  10.2172/1022154
RP-ID  :  LLNL-TR-479311
RP-ID  :  W-7405-ENG-48
RP-ID  :  1022154

美国|英语

来源: UNT Digital Library

【 摘 要 】

Genetic manipulations in bacteria currently rely on the introduction of antibiotic resistance genes into a bacterial strain; for those organisms that will be used for commercial or industrial applications, the genetic cassette encoding the antibiotic resistance is sometimes removed after selection. it is clear that if alternative technologies could obviate the need to introduce antibiotic resistance into bacteria, they would most certainly become a standard tool in molecular micriobiology for commercial, industrial as well as research applications. Here, they present the development of a novel genetic engineering technology based on toxin-antitoxin systems to modify bacterial genomes without the use of antibiotic resistance in the mutagenesis process. The primary goal is to develop antibiotic-free selection for genetically altered select agent pathogens. They are adapting the toxinc-antitoxin system to enable gene replacement in select agent pathogens since the NIH restrictions introducing antibiotic resistance into select agent pathogens have hindered research with select agent pathogens.