Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil

Revista do Instituto de Medicina Tropical de São Paulo

Pedro Alves D'azevedo²  Guilherme H.c. Furtado¹  Eduardo A.s. Medeiros¹  Kelly Aline Santiago²  Suzane Silbert²  Antonio C.c. Pignatari² 

[1] ,Universidade Federal de São Paulo Laboratório Especial de Microbiologia Clínica São Paulo SP ,Brasil

关键词: VRE;    E. faecalis;    E. faecium;    PFGE ("Pulsed-Field Gel Electrophoresis");   

DOI  :  10.1590/S0036-46652008000400001

来源: SciELO

【 摘 要 】

E. faecium was the first reported VRE species, carrying the vanA gene in Brazil. In spite of this, vancomycin-resistant E. faecalis has become the predominant species in Brazilian hospitals. The aim of this study was to evaluate the genetic relatedness of VREs isolated in a Brazilian teaching hospital eight years apart from its first isolation. We analyzed 38 VRE strains obtained from 81 surveillance cultures of patients admitted to the four largest intensive care units in Hospital São Paulo in February, 2006. Presence of the vanA gene was assayed by PCR and PFGE analysis was used for molecular characterization. All VRE strains carried the vanA gene. Two distinct clonal groups were observed among vancomycin-resistant E. faecalis. Vancomycin-resistant E. faecium belonged to five distinct clones were demonstrated by molecular typing. All of these clones were different from the first vancomycin-resistant enterococci clone isolated eight years ago in our hospital.

【 授权许可】

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