Live Feeds Used in the Larval Culture of Red Cusk Eel, Genypterus chilensis, Carry High Levels of Antimicrobial-Resistant Bacteria and Antibiotic-Resistance Genes (ARGs)

Animals

MarkA. Añazco¹  FélixA. Godoy¹  Jaime Romero²  ClaudioD. Miranda³  Rodrigo Rojas³  Luz Hurtado⁴ 

[1] Centro i~mar, Universidad de Los Lagos, Puerto Montt 5480000, Chile;Laboratorio de Biotecnología, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Macul, Santiago 7810000, Chile;Laboratorio de Patobiología Acuática, Departamento de Acuicultura, Universidad Católica del Norte, Coquimbo 1780000, Chile;Programa Cooperativo de Doctorado en Acuicultura, Departamento de Acuicultura, Universidad Católica del Norte, Coquimbo 1780000, Chile;

关键词: resistant bacteria;    red cusk eel;    genypterus chilensis;    florfenicol;    vibrios;    live feed;    rotifer;    artemia;    flor;    fexa;   

DOI  :  10.3390/ani10030505

来源: DOAJ

【 摘 要 】

The culture of red cusk eel Genypterus chilensis is currently considered a priority for Chilean aquaculture but low larval survival rates have prompted the need for the continuous use of antibacterials. The main aim of this study was to evaluate the role of live feed as a source of antibacterial-resistant bacteria in a commercial culture of G. chilensis. Samples of rotifer and Artemia cultures used as live feed were collected during the larval growth period and culturable bacterial counts were performed using a spread plate method. Rotifer and Artemia cultures exhibited high levels of resistant bacteria (8.03 × 10⁴ to 1.79 × 10⁷ CFU/g and 1.47 × 10⁶ to 3.50 × 10⁸ CFU/g, respectively). Sixty-five florfenicol-resistant isolates were identified as Vibrio (81.5%) and Pseudoalteromonas (15.4%) using 16S rRNA gene sequence analysis. A high incidence of resistance to streptomycin (93.8%), oxytetracycline (89.2%), co-trimoxazole (84.6%), and kanamycin (73.8%) was exhibited by resistant isolates. A high proportion of isolates (76.9%) carried the florfenicol-resistance encoding genes floR and fexA, as well as plasmid DNA (75.0%). The high prevalence of multiresistant bacteria in live feed increases the incidence of the resistant microbiota in reared fish larvae, thus proper monitoring and management strategies for live feed cultures appear to be a priority for preventing future therapy failures in fish larval cultures.

【 授权许可】

Unknown