Vaccination with human induced pluripotent stem cells creates an antigen-specific immune response against HIV-1 gp160

Frontiers in Microbiology

Naoki eYamamoto¹  Mayuko eNishi²  Shinji eYoshizaki²  Akihide eRyo²  Yoshitsugu eKojima²  Asami eKondo² 

[1] National University of Singapore;Yokohama City University;

关键词: HIV;    Vaccine;    aids;    Cellular immunity;    iPS cells;    Ionizing radiation;   

DOI  :  10.3389/fmicb.2011.00027

来源: DOAJ

【 摘 要 】

Induced pluripotent stem cells (iPSCs) are artificially derived from somatic cells that have been transduced with defined reprogramming factors. A previous report has indicated the possibility of using iPSCs as an immune stimulator to generate antigen-specific immunity. In our current study, we have investigated whether human iPSCs (hiPSCs) have the ability to enhance specific immune response against a human immunodeficiency virus type 1 (HIV-1) antigen in a xenogenic mouse model. Our results show that BALB/c mice immunized with hiPSCs transduced with an adenoviral vector encoding HIV-1 gp160 exhibited prominent antigen-specific cellular immune responses. We further found that pre-treatment of hiPSCs with ionizing radiation promotes the secretion of pro-inflammatory cytokines such as interleukin-1 alpha (IL-1α), IL-12 and IL-18. These cytokines might promote the activation of antigen-presenting cells and the effective induction of cellular immunity. Our present findings thus demonstrate that a hiPSCs-based vaccine has the potential to generate cellular immunity against viral antigens such as HIV-1 gp160 in a xenogenic condition.

【 授权许可】

Unknown