Liposome oligomannose‐coated with neoglycolipid, a new candidate for a safe adjuvant for induction of CD8+ cytotoxic T lymphocytes

FEBS Letters

Yamamoto, Naoki³  Hatanaka, Masakazu³  Sakakibara, Ryuzo²  Mizuochi, Tsuguo¹  Shimizu, Yoshitaka¹  Fukasawa, Masashi³  Nakata, Munehiro¹  Shikata, Kohdoh¹ 

[1] Laboratory of Biomedical Chemistry, Department of Applied Chemistry, Tokai University, Hiratsuka, Kanagawa 259-1292, Japan;Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University, Bunkyo-machi, Nagasaki 852-8521, Japan;Department of Preventive Medicine, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki 852-8102, Japan

关键词: Neoglycolipid;    Vaccine;    Adjuvant;    Cellular immunity;    Cytotoxic T lymphocyte;    Human immunodeficiency virus;    CTL;    cytotoxic T lymphocyte;    HIV;    human immunodeficiency virus;    Man;    mannose;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(98)01577-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cytotoxic T lymphocyte (CTL) response has recently been shown to play a role in protection against human immunodeficiency virus (HIV) and it is therefore thought that a vaccine against HIV must be able to elicit a CTL response. The development of a safe, effective adjuvant is very important because alum, the only adjuvant available for use in humans at present, can barely induce a response of this type. We demonstrate here that liposomes that contain an immunodominant peptide (15 amino acids) of the envelope glycoprotein gp120 of HIV-1 and that are coated with mannopentaose-dipalmitoylphosphatidylethanolamine conjugate induce a major histocompatibility complex class I-restricted CD8⁺ CTL response in mice with a single subcutaneous immunization, whereas non-coated liposomes do not. Since no damage to the skin at the injection site was caused by the liposomes, and since the oligomannose-coated liposomes consist of innocuous materials ubiquitously distributed throughout the human body, they may be highly suitable for use as a safe adjuvant in vaccines inducing a CTL response against HIV.

【 授权许可】

Unknown