Optimized protocol for double vaccine immunization against classical swine fever and porcine reproductive and respiratory syndrome

BMC Veterinary Research

Research

Shouhua Feng¹  Lixia Liu¹  Chao Ni¹  Ziyu Liu¹  Changde Wu¹  Jinling Liu¹  Wanting Liu¹  Baiqiang Shan¹  Zeliang Chen¹  Xiaoli Wang¹  Shu Wei²  Hongtao Wu² 

[1] Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, China, No.120, Dongling Road, Shenhe District, 110866, Shenyang, People’s Republic of China;The Preventive Center of Animal Disease of Liaoning Province, Liaoning Agricultural Development Service Center, No.95, Renhe Road, Shenbei District, 110164, Shenyang, People’s Republic of China;

关键词: Classical swine fever;    Porcine reproductive and respiratory syndrome;    Vaccine;    Humoral immunity;    Cellular immunity;   

DOI  :  10.1186/s12917-022-03559-z

 received in 2022-05-19, accepted in 2022-12-21,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundClassical swine fever and porcine reproductive and respiratory syndrome have seriously affected the development of the swine breeding industry in China. Vaccine immunization remains the main way to prevent these infections. The aim of this study was to establish an optimized protocol for vaccine immunization against classical swine fever virus (CSFV) and porcine reproductive and respiratory syndrome virus (PRRSV).MethodsBlood samples were collected from the anterior vena cava of pigs after immunization, and blood indices, secreted levels of specific antibodies and neutralizing antibodies associated with humoral immunity, the proliferation capacity of T lymphocytes as a measure of cellular immunity, and secreted levels of IFN-γ and TNF-α were determined.ResultsThe results showed that simultaneous immunization against CSFV and PRRSV infections induced strong and specific humoral and T-cellular immune responses, high levels of cytokine IFN-γ secretion and delayed secretion of cytokine TNF-α. Moreover, significantly higher lymphocyte percentages and red blood cell and leukocyte counts were found in the group simultaneously immunized against CSFV and PRRSV. However, no statistically significant differences were observed in hemoglobin values, neutrophil counts, and median cell percentages among the S + PRRS, PRRS-S, and S-PRRS groups.ConclusionThis study demonstrated that simultaneous immunization against CSFV and PRRSV had the advantages of inducing a rapid, enhanced, and long-lasting immune response. These findings provide a theoretical basis for the establishment of a reasonable and optimized vaccine immunization protocol against CSFV and PRRSV in combination with a variety of other vaccine inoculations.

【 授权许可】

CC BY   
© The Author(s) 2023

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