The Effect of Chlorogenic Acid on Bacillus subtilis Based on Metabolomics

Molecules

Min Zhang¹  Zhenhua Wang¹  Xin Ren¹  Shan Liang¹  Yan Wu¹  Ziyuan Wang¹ 

[1] Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China;

关键词: chlorogenic acid;    Bacillus subtilis;    antimicrobial activity;    metabolomics;   

DOI  :  10.3390/molecules25184038

来源: DOAJ

【 摘 要 】

Chlorogenic acid (CGA), a natural phenolic compound, is an important bioactive compound, and its antibacterial activity has been widely concerned, but its antibacterial mechanism remains largely unknown. Protein leakage and the solution exosmosis conductivity of Bacillus subtilis 24434 (B. subtilis) reportedly display no noticeable differences before and after CGA treatment. The bacterial cells treated with CGA displayed a consistently smooth surface under the electron microscope, indicating that CGA cannot directly disrupt bacterial membranes. However, CGA induced a significant decrease in the intracellular adenosine triphosphate (ATP) concentration, possibly by affecting the material and energy metabolism or cell-signaling transduction. Furthermore, metabolomic results indicated that CGA stress had a bacteriostatic effect by inducing the intracellular metabolic imbalance of the tricarboxylic acid (TCA) cycle and glycolysis, leading to metabolic disorder and death of B. subtilis. These findings improve the understanding of the complex action mechanisms of CGA antimicrobial activity and provide theoretical support for the application of CGA as a natural antibacterial agent.

【 授权许可】

Unknown