Antimicrobial Activity Investigation on Wuyiencin Fractions of Different Polarity

Molecules

Zengjie Cui¹  Kecheng Zhang¹  Gaimei She¹  Yinni Lin¹  Lei Sun¹  Yan Cheng¹ 

[1] ¹State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China ²School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China ³Department of Biotechnology, Beijing City University, Beijing, 100094, China

关键词: Wuyiencin;    antimicrobial activity;    Rhodotorula rubra;    Bacillus subtilis;    Bacillus megaterium;    Escherichia coli;    Cladosporium fulvum;    Staphylococcus aureus;   

DOI  :  10.3390/molecules15053179

来源: mdpi

【 摘 要 】

The aim of this study was to evaluate the antimicrobial activity of Wuyiencin fractions with different polarities against six indicator microorganisms: Rhodotorula rubra, Bacillus subtilis, Bacillus megaterium, Escherichia coli, Cladosporium fulvum and Staphylococcus aureus. The fermentation broth of Wuyiencin was submitted to AB-8 macroporous adsorptive resin and fractionated with solvents of different polarity. The fraction eluted with water had remarkably antimicrobial activity against all the microorganisms investigated except for C. fulvum and S. aureus (MIC ≤ 0.0625 mg/mL), probably due to the presence of active components. The fraction eluted with methanol showed potential antimicrobial activity against all the test microorganisms except for R.rubra, with MIC values of0.5 and 2 mg/mL. In conclusion, fractions eluted with water and methanol, respectively, represent the main active-part of Wuyiencin, and could be emphasized for agricultural applications in the future.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.