Chemical Diversity and Antimicrobial Potential of Cultivable Fungi from Deep-Sea Sediments of the Gulf of Mexico

Molecules

Meritxell Riquelme¹  Carlos A. Fajardo-Hernández²  Alejandra Arista-Romero²  Mario Figueroa²  Anahí Martínez-Cárdenas²  Rodrigo Villanueva-Silva²  Scott G. Franzblau³  Rui Ma³  Mallique Qader³  Baojie Wan³  Patricia Velez⁴ 

[1] Departamento de Microbiología, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Ensenada, Baja California 22860, Mexico;Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico;Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA;Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico;

关键词: marine fungi;    deep-sea sediments;    chemical diversity;    metabolomics;    antimicrobial activity;   

DOI  :  10.3390/molecules26237328

来源: DOAJ

【 摘 要 】

A collection of 29 cultivable fungal strains isolated from deep-sea sediments of the Gulf of Mexico were cultivated under the “one strain, many compounds” approach to explore their chemical diversity and antimicrobial potential. From the 87 extracts tested, over 50% showed antimicrobial activity, and the most active ones were those from cultures grown at 4 °C in darkness for 60 days (resembling deep-sea temperature). PCA analysis of the LC-MS data of all the extracts confirmed that culture temperature is the primary factor in the variation of the 4462 metabolite features, accounting for 21.3% of the variation. The bioactivity-guided and conventional chemical studies of selected fungal strains allowed the identification of several active and specialized metabolites. Finally, metabolomics analysis by GNPS molecular networking and manual dereplication revealed the biosynthetic potential of these species to produce interesting chemistry. This work uncovers the chemical and biological study of marine-derived fungal strains from deep-sea sediments of the Gulf of Mexico.

【 授权许可】

Unknown