Controlled Delivery of Stem Cell‐Derived Trophic Factors Accelerates Kidney Repair After Renal Ischemia‐Reperfusion Injury in Rats

Stem Cells Translational Medicine

Hyung Eun Yim¹  Sunil K. George¹  In Kap Ko¹  Michael W. Kim¹  Brian Shing¹  James J. Yoo¹  Doo Sang Kim¹  Zachary Atala¹  Ji Hyun Kim¹  Hyun Chul Chung¹  Kyung Hyun Moon¹ 

[1] Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine Medical Center Boulevard Winston‐Salem North Carolina USA;

关键词: Acute kidney injury;    Conditioned medium;    Drug delivery systems;    Kidney regeneration;   

DOI  :  10.1002/sctm.18-0222

来源: DOAJ

【 摘 要 】

Abstract Renal disease is a worldwide health issue. Besides transplantation, current therapies revolve around dialysis, which only delays disease progression but cannot replace other renal functions, such as synthesizing erythropoietin. To address these limitations, cell‐based approaches have been proposed to restore damaged kidneys as an alternative to current therapies. Recent studies have shown that stem cell‐derived secretomes can enhance tissue regeneration. However, many growth factors undergo rapid degradation when they are injected into the body in a soluble form. Efficient delivery and controlled release of secreting factors at the sites of injury would improve the efficacy in tissue regeneration. Herein, we developed a gel‐based delivery system for controlled delivery of trophic factors in the conditioned medium (CM) secreted from human placental stem cells (HPSCs) and evaluated the effect of trophic factors on renal regeneration. CM treatment significantly enhanced cell proliferation and survival in vitro. Platelet‐rich plasma (PRP) was used as a delivery vehicle for CM. Analysis of the release kinetics demonstrated that CM delivery through the PRP gel resulted in a controlled release of the factors both in vitro and in vivo. In an acute kidney injury model in rats, functional and structural analysis showed that CM delivery using the PRP gel system into the injured kidney minimized renal tissue damage, leading to a more rapid functional recovery when compared with saline, CM, or vehicle only injection groups. These results suggest that controlled delivery of HPSC‐derived trophic factors may provide efficient repair of renal tissue injury. Stem Cells Translational Medicine 2019;8:959&970

【 授权许可】

Unknown