Comparison of fibroblast cell regeneration in three different concentrations of Wharton's Jelly mesenchymal stem cells conditioned medium (WJMSCs-CM)

1st Physics and Technologies in Medicine and Dentistry Symposium

物理学;医药卫生

Untoro, E.G.^1 ; Asrianti, D.^1 ; Usman, M.^1 ; Meidyawati, R.^1 ; Margono, A.^1

Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia^1

关键词: Alternative source;    Conditioned medium;    Control groups;    Extracellular matrices;    Fibroblast cells;    Fibroblast proliferation;    Mesenchymal stem cell;    Treatment group;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/884/1/012067/pdf
DOI  :  10.1088/1742-6596/884/1/012067

学科分类：卫生学

来源: IOP

【 摘 要 】

Wharton's Jelly-derived mesenchymal stem cells (WJMSCs) have gained interest as an alternative source of stem cells for regenerative medicine. Although many studies have characterized Wharton's Jelly biologically, the effects of different concentrations in a cultured medium have not yet been compared. Damaged fibroblasts, the primary components of irreversible dental pulpitis, irreversibly impair the ability to regenerate and lead to the disruption of extracellular matrix. This study was performed to evaluate the potency of three WJMSCs-CM concentrations in improving serum-starved fibroblasts. Fibroblasts were cultivated in five passages, and divided into four groups. The first group (the control group) consisted of fibroblast cells that had been treated using starvation methods. The other groups (the treatment groups) were treated with various concentration of WJMSCs-CM (50%, 25% and 12.5%). Proliferative ability was evaluated using a cell count method and analyzed with a one-way ANOVA. Cultivation of serum-starved fibroblasts produced significantly higher cell counts in 12.5% WJMSCs-CM compared to the 50% group. It can be concluded that 12.5% WJMSCs-CM is the most efficient concentration for fibroblast proliferation.