The Research of Acellular pancreatic bioscaffoldas a natural 3D platform In Vitro

2017 International Symposium on Application of Materials Science and Energy Materials

材料科学;能源学

Wang, Xin^1 ; Li, Zhao^2

School of Tianjin Medical University, Tianjin, China^1

School of Peking University, Beijing, China^2

关键词: Biomaterial scaffolds;    Extracellular matrices;    Functional properties;    Histological structure;    Immunological response;    Pancreatic acinar cells;    Protein expressions;    Vascular endothelial cells;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/322/2/022044/pdf
DOI  :  10.1088/1757-899X/322/2/022044

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

AIM: To investigate the biochemical and functional properties of a rat acellular pancreatic bioscaffold (APB). METHODS: Fresh pancreata were soaked and perfused. The histological structure, the extracellular matrix (ECM) composition, and the DNA content of the APBs were evaluated. After biocompatibility studies, the proliferation, apoptosis and differentiation of AR42J pancreatic acinar cells cultured on APBs were assessed. RESULTS: The pancreatic tissues became translucent after decellularization. The native macroscopic 3D architecture and the ECM ultrastructure were preserved, with large ductal structures and vascular tissue branching from the greater pancreatic artery, but there were no visible vascular endothelial cells, cellular components or cracked cellular debris. The ECM components, including collagen I, collagen IV, fibronectin, laminin and sGAG, were not decreased after decellularization of the APB (P>0.05); however, the DNA content was decreased significantly (P