Combination of lyophilized adipose-derived stem cell concentrated conditioned medium and polysaccharide hydrogel in the inhibition of hypertrophic scarring

Stem Cell Research & Therapy

Liangwan Chen¹  Li Zhang²  Jinghua Chen³  Penghong Chen⁴  Xiaosong Chen⁴  Guohao Peng⁴  Haoruo Zhang⁴  Ming Li⁴  Shijie Tang⁴  Haiyan Weng⁴  Chaoyu Zhang⁴  Ting Wang⁴  Aizhen Chen⁴  Hangqi Gao⁴  Shirong Li⁵ 

[1] Department of Cardiac Surgery, Fujian Medical University Union Hospital;Department of Central Sterile Supply, Fujian Medical University Union Hospital;Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University;Department of Plastic Surgery, Fujian Medical University Union Hospital;Department of Plastic and Reconstructive Surgery, Southwestern Hospital, Army Military Medical University;

关键词: Adipose-derived stem cells;    Conditioned medium;    Freeze-drying;    Hydrogel;    Scar hyperplasia;   

DOI  :  10.1186/s13287-020-02061-3

来源: DOAJ

【 摘 要 】

Abstract Background Mesenchymal stem cell-based acellular therapies have been widely exploited in managing hypertrophic scars. However, low maintenance dose and transitory therapeutic effects during topical medication remain a thorny issue. Herein, this study aimed to optimize the curative effect of adipose-derived stem cell conditioned medium (ADSC-CM) in the prevention of hypertrophic scarring. Methods In the present study, ADSC-CM was concentrated via the freeze-drying procedure. The efficacy of different dose groups (CM, CM5, CM10) was conducted on the proliferation, apoptosis, and α-smooth muscle actin (α-SMA) expression of human keloid fibroblasts (HKFs) in vitro. Incorporation of adipose-derived stem cell concentrated conditioned medium (ADSCC-CM) into polysaccharide hydrogel was investigated in rabbit ear, in vivo. Haematoxylin-eosin (H&E) and Masson’s trichrome staining were performed for the evaluation of scar hyperplasia. Results We noted that ADSCC-CM could downregulate the α-SMA expression of HKFs in a dose-dependent manner. In the rabbit ear model, the scar hyperplasia in the medium-dose group (CM5) and high-dose group (CM10) was inhibited with reduced scar elevation index (SEI) under 4 months of observation. It is noteworthy that the union of CM5 and polysaccharide hydrogel (CM5+H) yielded the best preventive effect on scar hyperplasia. Briefly, melanin, height, vascularity, and pliability in the CM5+H group were better than those of the control group. Collagen was evenly distributed, and skin appendages could be regenerated. Conclusions Altogether, ADSCC-CM can downregulate the expression of α-SMA due to its anti-fibrosis effect and promote the rearrangement of collagen fibres, which is integral to scar precaution. The in situ cross bonding of ADSCC-CM and polysaccharide hydrogel could remarkably enhance the therapeutic outcomes in inhibiting scar proliferation. Hence, the alliance of ADSCC-CM and hydrogel may become a potential alternative in hypertrophic scar prophylaxis.

【 授权许可】

Unknown