The influence of freezing rates on bovine pericardium tissue Freeze-drying

Brazilian Archives of Biology and Technology

Camila Figueiredo Borgognoni¹  Virgilio Tattini Junior¹  Ana Maria Irene Bartolomeu Ayrosa¹  Bronislaw Polakiewicz¹  Adolfo Alberto Leirner²  Marina Junko Shiotsu Maizato²  Olga Zazuco Higa²  Marisa Masumi Beppu²  Ronaldo Nogueira De Moraes Pitombo¹ 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas São Paulo SP ,Brasil

关键词: Freeze-drying;    freezing;    bovine pericardium;   

DOI  :  10.1590/S1516-89132009000600021

来源: SciELO

【 摘 要 】

The bovine pericardium has been used as biomaterial in developing bioprostheses. Freeze-drying is a drying process that could be used for heart valve's preservation. The maintenance of the characteristics of the biomaterial is important for a good heart valve performance. This paper describes the initial step in the development of a bovine pericardium tissue freeze-drying to be used in heart valves. Freeze-drying involves three steps: freezing, primary drying and secondary drying. The freezing step influences the ice crystal size and, consequently, the primary and secondary drying stages. The aim of this work was to investigate the influence of freezing rates on the bovine pericardium tissue freeze-drying parameters. The glass transition temperature and the structural behaviour of the lyophilized tissues were determined as also primary and secondary drying time. The slow freezing with thermal treatment presented better results than the other freeze-drying protocols.

【 授权许可】

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