Mechanical, thermal and morphological properties of glutaraldehyde crosslinked bovine pericardium followed by glutamic acid treatment

Materials Research

Gilberto Goissis²  Domingo Marcolino Braile²  Nélly Cristina Carnevalli¹  Vladimir Aparecido Ramirez² 

[1] ,Braile Biomédica Ind. Com. e Repres. LtdaSão José do Rio Preto SP ,Brazil

关键词: bovine pericardium;    glutaraldehyde;    crosslinking;    glutamic acid;    properties;   

DOI  :  10.1590/S1516-14392009000100015

来源: SciELO

【 摘 要 】

Major problems with valve bioprostheses are associated with progressive structural deterioration and calcification, directly associated with the use of glutaraldehyde (GA). This work describes the effects of GA processing and borate/glutamic acid buffer treatment on the mechanical, thermal and morphological properties of 0.5% GA crosslinked bovine pericardium (BP). The results showed that while the treatment of 0.5% GA crosslinked BP with borate/glutamic acid significantly improves the mechanical properties, it had no visible effect on surface morphology. Better surface preservation was only achieved for BP pre-treated with a lower GA concentration followed by the conventional treatment (0.5% GA). Improvements in mechanical properties probably arises from structural changes probably involving the depolymerization of polymeric GA crosslinks and an increase electrostatic interaction due to covalent binding of glutamic acid to free carbonyl groups (Schiff base).The results indicate that the treatment GA crosslinked BP with borate/glutamic acid buffer may be an attractive procedure for the manufacture of heart valve bioprostheses.

【 授权许可】

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