Characterization of Maize Superoxide Dismutase Gene Family: Identification of Two Novel Transcripts of Chloroplast SODs and Analysis of Potential DNA-protective Function of Mitochondrial MnSOD

Journal of Stress Physiology & Biochemistry

关键词: reactive oxygen species;    superoxide dismutase;    chloroplasts;    mitochondria;   

DOI  :  

来源: DOAJ

【 摘 要 】

The families of genes encoding such key antioxidant enzymes as superoxide dismutases usually havecomplexstructure.Theyconsistofgenesencodingenzymeswhichdifferbytheirmetal cofactor, origin and subcellular localization. The maize SOD family consisting of multiple genes is one of the most interesting among the characterized plant SOD gene families. We re-investigated the structure of maize SOD gene family using different approaches. We revealed two novel gene transcripts encoding chloroplast Fe- and Cu/Zn-SOD. This finding indicates that the reactive oxygen species (ROS) detoxication system in maize chloroplasts has more complicated organization than it was considered earlier. At the same time it appeared that the number of maize genes encoding mitochondrial Mn-SODs is less than it was supposed. Using the recombinant proteins we showed thatthefunctioningofMnSODenzymesisposttranslationallyregulatedby phosphorylation/dephosphorylation.PossibleinvolvementofMn-SODintheprotectionof mitochondrial DNA from oxidative damage is investigated.

【 授权许可】

Unknown